E oona le dihydrocoumarin

Dihydrocoumarin, manogi, faʻaaogaina i meaʻai, faʻaaogaina foi e fai ma sui o le coumarin, faʻaaogaina e fai ma mea manogi; Faafefiloi kulimi, popo, tofo o le kinnamon; E fa'aaogaina fo'i e fai ma tofo tapaa.

E oona le dihydrocoumarin

Dihydrocoumarin e le oona. O le Dihydrocoumarin o se oloa fa'anatura e maua i le samasama vanilla rhinoceros. E saunia e ala i le hydrogenation o le coumarin i le i ai o le nickel catalyst i le 160-200 ℃ ma lalo o le mamafa. E mafai foi ona faʻaaogaina e pei o mea mata, hydrolyzed i alkaline vai vaifofo e maua ai le o-hydroxyphenylpropionic acid, dehydration, tapuni-mataloa maua.

Tulaga teuina

Tapuni ma pogisa, teuina i se nofoaga malulu ma mago, o le avanoa i totonu o le paelo e laʻititi pe a mafai i lalo o pemita saogalemu, ma faʻatumu i le puipuiga o le nitrogen. Teu i totonu o se faleteuoloa malulu ma ea. Aloese mai le afi, vai. E tatau ona teuina ese mai le oxidizer, aua le faʻafefiloi le teuina. Fa'aauupegaina i le ituaiga tutusa ma le tele o mea afi afi.

Su'esu'ega in vitro

In vitro enzymatic assay, dihydrocoumarin fa'aoso fa'atonuga fa'alagolago fa'asaina o le SIRT1 (IC50 o le 208μM). Fa'aitiitiga i le SIRT1 deacetylase gaoioiga na matauina e oo lava i micromolar doses (85 ± 5.8 ma 73 ± 13.7% gaioiga i 1.6μM ma 8μM, faasologa). O le Microtubule SIRT2 deacetylase na faʻalavelaveina foi i se auala faʻalagolago i fua (IC50 o le 295μM).

A maeʻa le 24 itula o le faʻaalia, o le dihydrocoumarin (1-5mM) faʻateleina le cytotoxicity i laina telefoni TK6 i se fua faʻalagolago. Dihydrocoumarin (1-5mM) faʻateleina apoptosis i TK6 laina sela i se fua faʻalagolago i le 6-itula taimi taimi. O le 5mM dose o le dihydrocoumarin na faʻateleina apoptosis i le 6-itula taimi taimi i le TK6 cell line. A maeʻa le 24-itula taimi faʻaalia, dihydrocoumarin (1-5mM) faʻateleina le p53 lysine 373 ma le 382 acetylation i se fua faʻalagolago i le TK6 cell line.